Journal: Translational Oncology

Article Title: CD169-positive macrophages enhance abscopal effect of radiofrequency ablation therapy in liver cancer

doi: 10.1016/j.tranon.2021.101306

Figure Lengend Snippet: CD169+ macrophages repress tumor progression in liver. (A-D) B16-F10 melanoma cells grew faster in CD169-DTR mice. (A) Experimental design. (B) Growth of tumors was examined using in vivo imaging system by evaluating abdominal fluorescence signals. The average radiance of two groups were compared. Two-way ANOVA with Sidak's multiple comparisons test. (C) The livers bearing with metastatic tumors were harvested (left panel). The number of tumors in each liver were counted (right panel). (D) Representative H&E staining of liver tissues. Scale bar, 200μm, 50μm. The data were from one experiment representative of two independent experiments. (E-G) Transfer of CD169+ macrophages inhibits B16-F10 melanoma cells growth in CD169-DTR mice. (E) Experiment design (n = 3∼4 per group). (F) The abdominal fluorescence signals were examined with in vivo imaging system. The average radiance of two groups were compared. (G) The livers were harvested from the mice receiving CD169+ macrophages or vehicle, and number of tumors were counted.

Article Snippet: To monitor progression of liver metastases, intraperitoneal injection of D-Luciferin potassium salt (Beyotime Biotechnology, Shanghai, China) was executed 5 min before bioluminescent imaging within the IVIS Spectrum System (PerkinElmer, MA, USA).

Techniques: In Vivo Imaging, Fluorescence, Staining